Original article / research
Year : 2023 Month : April Volume : 12 Issue : 2 Page : MO18 - MO23 Full Version

Bacteriological Profile and Antimicrobial Susceptibility Pattern of the Isolates from Body Fluid Samples from Tertiary Care Health Centre, Ujjain, Madhya Pradesh, India
Sonali Waske, Yogyata Marothi
1. Assistant Professor, Department of Microbiology, R.D. Gardi Medical College, Ujjain, Madhya Pradesh, India. 2. Professor and Head, Department of Microbiology, R.D. Gardi Medical College, Ujjain, Madhya Pradesh, India
 
Correspondence Address :
Sonali Waske, Yogyata Marothi,
Sonali Waske,
RDGMC Ujjain, Madhya Pradesh, India.
E-mail: drsonalip@gmail.com
 
ABSTRACT
: Introduction: Bacterial Infection of various body fluids can leads to serious invasive infection, high morbidity and mortality. It is a clinical urgency; so early detection and identification of pathogen is essential for appropriate management of patient.

Aim: To determine the bacterial profile and their susceptibility to antimicrobial agents of isolates from various body fluids.

Materials and Methods: This retrospective cross-sectional study was conducted from January, 2021 to December 2021 in the Microbiology Department, CRG Hospital, RD Gardi Medical College, Ujjain, Madhya Pradesh, India. Body fluids were collected and transported aseptically from the patients visited in this hospital. All received samples were processed as per standard microbiology methods. Antimicrobial susceptibility testing was performed using the Kirby-Bauer disk diffusion method and interpreted as per Clinical and Laboratory Standards Institute (CLSI) guidelines.

Results: A total of 298 body fluids samples were received for culture and sensitivity, of these, 63 (21.1%) fluid samples showed growth. Out of them, maximum pathogens were isolated from Broncho Alveolar Lavage (BAL) fluid 28 (44.4%), followed by peritoneal fluid 17 (27%), pleural fluid 12 (19%), CSF 1 (1.6%), pericardial fluid 1 (1.6%) and synovial 4 (6.3%). Most of the isolates were Gram-Negative Bacteria (GNB) 58 (92%), predominantly Pseudomonas aeruginosa 20 (32%) followed by Klebsiella species 18 (29%), E.coli 14 (22%), All gram negative isolates were sensitive to Tigecycline and Colistin, However, susceptibility to the carbapenem group (Imipenem, Miropenem and Ertapenem) were (80-100%). Among gram positive bacteria, Staphylococcus aureus were 5 (8%) isolated. Of these, Methicillin Resistant Staphylococcus aureus (MRSA) was 4 (6.3%) and Methicillin Sensitive Staphylococcus Aureus (MSSA) was 1 (1.6%). All isolate of Staphylococcus aureus were susceptible to Vancomycin, Linezolide, Teicoplanin and Levofloxacin.

Conclusion: Knowledge of bacteriological and antimicrobial profile of body fluids is necessary, so that the life threatening infections can be effectively treated. In this study, BAL fluid is the commonest received sample. GNB were main isolates among them Pseudomonas aeruginosa is frequently isolated pathogen. All isolated pathogens were susceptible for higher group of antimicrobial agents (tigecycline, colistin) and showed reduced sensitivity towards other antibiotics. So, regular monitoring of the pattern of Antimicrobial Resistance (AMR) of bacteriological isolates in the patients is critical to develop antibiotic policy to combat these infections and reduce morbidity and mortality.
Keywords : Antimicrobial resistance, Bronchoalveolar lavage, Gram-negative bacteria, Gram-positive bacteria
DOI and Others : DOI: 10.7860/NJLM/2023/59624.2726 Date of Submission: Aug 12, 2022 Date of Peer Review: Sep 24, 2022 Date of Acceptance: Nov 23, 2022 Date of Publishing: Apr 01, 2023 AUTHOR DECLARATION: • Financial or Other Competing Interests: None • Was Ethics Committee Approval obtained for this study? NA • Was informed consent obtained from the subjects involved in the study? NA • For any images presented appropriate consent has been obtained from the subjects. NA PLAGIARISM CHECKING METHODS
 
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