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| Year:
2014 |
Month:
June
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Volume:
3 |
Issue:
2 |
Page:
22 - 27 |
Phenotypic Tests for Detecting Incidence of Metallo-Β-Lactamase (Mbl ) Producing Pseudomonas aeruginosa in Jaipur
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Correspondence
Smita Sood,
Dr. Smita Sood
3Kha 4 A, Jawahar Nagar, Jaipur, India.
E-mail : drsmitasood@yahoo.co.in :
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Background: Metallo-beta-lactamase (MBL) mediated resistance to carbapenems is an emerging threat in hospital isolates of Pseudomonas aeruginosa.Prompt detection and recognition of MBLs is important for timely implementation of infection control interventions and treatment with alternative antimicrobials. There is limited information from the state of Rajasthan regarding the prevalence of such enzymes.
Objectives: A study was conducted to determine the prevalence of metallo – β – lactamases (MBLs) in clinical strains of Psuedomonas aeruginosa and to compare three phenotypic tests as a method for MBL detection.
Material And Methods: Pseudomonas aeruginosa strains isolated from various clinical specimens at a tertiary care private hospital in Jaipur city, which tested resistant to imipenem, were tested for MBL production using three phenotypic tests: double disc synergy test, disc potentiation test and thiol based compound test.
Result: Of the 95 Pseudomonas aeruginosa isolates obtained during the study period, 69 (72.63%) were resistant to imipenem. All the three phenotypic methods were equally effective in demonstrating that 100% of the imipenem resistant strains were harboring MBLs. Maximum MBL producer isolates were found in patients admitted to the ICUs (56.52%) with majority of isolates from the Medical ICU (47.82%). Maximum MBL producers were obtained from lower respiratory secretions (39.13%) followed by urine (27.52%). 100% sensitivity to Colistin and Polymixin B was observed in these MBL harboring Pseudomonas aeruginosa.
Conclusion: The three phenotypic methods proved to be equally effective as a method to detect MBL production in Pseudomonas aeruginosa isolates and any one of these can be easily incorporated in routine lab procedures to detect MBLs.
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