Original article / research
To Identify the Prevalence Leptospira Serogroups in the Cases From Southern Gujarat Region
Dr. Tanvi Panwala,
E-7, Vighneshwar Estate, Nanpura,
Timaliavad, Surat-395001, Gujrat, India.
Introduction: Leptospirosis, is a widespread zoonosis which affects the human worldwide. It is an acute febrile illness; the severity varies from mild to severely fatal form. Because of protean clinical manifestation and unavailability of appropriate laboratory diagnostic facilities in most part of the country, human cases has been reported less in India. Clinically leptospirosis shows, similar signs and symptoms with other diseases which mandate the need of laboratory confirmation.
Aim: To identify prevalent circulating serogroups in human cases of South Gujarat region by performing Microscopic Agglutination Test (MAT) and to compare results of commercially available IgM Enzyme-Linked Immunosorbent Assay (ELISA) with MAT.
Settings and Design: A retrospective cross sectional study.
Materials and Methods: A total of 157 serum samples were collected from suspected leptospirosis patients and subjected to serological tests like MAT and IgM-ELISA. Identification of predominant infecting serogroup was done by using panel of 11 serovars. Statistical Analysis Used: Sensitivity and specificity.
Results: MAT analysis of 157 samples showed 113(72%) to be positive. Antibodies were predominantly against serogroups like Leptospira autumnalis (60%), L. australis (16%) and L. pyrogenes (7%). IgM-ELISA showed a positivity of 74% compared to MAT.
Conclusion: L. Autumnalis is the predominant serovar identified in the present study so. MAT can be used as a gold standard in conjunction with an IgM-ELISA in diagnostic laboratories.
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