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Original article / research
Year : 2018 Month : April-June Volume : 7 Issue : 2 Page : MO01 - MO05

Prevalence of Onychomycosis in Hail Region, Saudi Arabia


Soha Abdallah Moursi, Razan AhmedAl Baqawi, Manal Naif Al shammari, Najah Shulaywih Al Enzi
1. Assistant Professor, Department of Microbiology, College of Applied Medical Sciences, Hail University, Saudi Arabia. 2. Student, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Hail University, Saudi Arabia. 3. Student, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Hail University, Saudi Arabia. 4. Student, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Hail University, Saudi Arabia.
 
Correspondence Address :
Dr. Soha Abdallah Moursi,
Assistant Professor, Department of Microbiology, College
of Applied Medical Sciences, Hail university,
PO: 2440, Saudi Arabia.
E-mail: Sohasma2011@gmail.com
 
ABSTRACT
: Dermatophytes, yeast and non dermatophyte molds causes onychomycosis, which is the fungal infection of the nail bed and skin beneath, have four clinical types: Distal subungual, proximal subungual, superficial and total dystrophic.

Aim: Isolation and identification of causative agents by conventional and molecular methods in order to examine the relative prevalence of dermatophytes, yeast and non dermatophyte molds among patients and correlate the clinical pattern to the agents.

Materials and Methods: From dermatology private clinics, beauty salons and female students of Hail University, adults(15-70 years old) crusts and nail clipping particles of 450 nail samples were collected from toe nails and finger nails in sterile petri dishes. Direct microscopic examination was applied with KOH 20% and gentle heating. Culture, API 32 CŪ and PCR-ITS, PCR-RFLP were carried out.

Results: Positive cases for fungal elements were 296 (65.8%), DLSO were the major type (50.6%), TDO (31.5%), PSO (10.5%) and SWO (1.7%). As causative agents Candida albicans were (33.1%), Trichophyton rubrum (18.2%), Trichophyton mentagrophytes complex (16.9%), mixed infection (14.5%), Epidermophyton floccosum (9.5%) and Aspergillus niger (7.8%).

Conclusion: Dermatophyteic onychomycosis were mostly found followed by yeast and non dermatophyte molds. Accurate identification of the causative agents help managing onychomycosis by giving the correct antifungal treatment, patients awareness prevent relapse and malformation of nails.
Keywords : Dermatophytes, Nail infection, Non dermatophyte molds, Yeast
How to cite this article :
Soha Abdallah Moursi, Razan AhmedAl Baqawi, Manal Naif Al shammari, Najah Shulaywih Al Enzi . PREVALENCE OF ONYCHOMYCOSIS IN HAIL REGION, SAUDI ARABIA. National Journal of Laboratoy Medicine [serial online] 2018 April [cited: 2018 Apr 26 ]; 7:MO01-MO05. Available from
http://njlm.net/back_issues.asp?issn=2277-8551&year=2018&month=April&volume=7&issue=2&page=MO01-MO05&id=2283  
 
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