Original article / research
Evaluation of Phenotypic Methods for Detection of Biofilm Formation in Uropathogens
Dr. T.A Dhanalakshmi,
Associate Professor, Department of Microbiology, AIMS, B.G.Nagara, Nagamangala Taluk, Mandya District-571448, B.G.Nagara, Karnataka, India.
Introduction: Urinary tract infection (UTI) is one of the leading causes of morbidity encountered in clinical practice. Biofilm produced by the urinary pathogens leads to recurrent and recalcitrant UTI there by contributing to longer stay in hospital and increased cost of treatment.
Aim: The present study was conducted to evaluate Congo Red Agar method (CRA) and Tube Method (TM) in detection of biofilm formation in uropathogens with respect to Tissue Culture Plate method (TCP).
Materials and Methods: Study was conducted in the Department of Microbiology from May 2016 to May 2017. Midstream clean catch urine collected from patients with symptoms of UTI was processed by standard methods. A total of 264 randomly selected urinary isolates were subjected to biofilm detection by CRA method, tube method and TCP method. TCP was considered as gold standard. Results were expressed in terms of percentages, sensitivity, specificity, Positive Predictive Value (PPV) and Negative Predictive Value (NPV).
Results: Biofilm was detected in 105 (39.77%) isolates by TCP method, in 124(46.97%) by CRA method and 101(38.26%) by tube method. CRA method and TM method showed a sensitivity of 80% & 63.81%, specificity of 75.47% & 78.62%, PPV of 68.29% & 66.34%, and NPV of 85.11% and 76.69% respectively.
Conclusion: Congo red agar and tube methods can be considered for detection of biofilms in resource constraint conditions.
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