Original article / research
Study on Drug Resistant Pulmonary Tuberculosis using Line Probe Assay in a Tertiary Care Hospital from Eastern India
Dr. Rohit Kumar,
Department of Microbiology, Indira Gandhi Institute of Medical Sciences (IGIMS), Patna-800014, Bihar, India.
Introduction: Pulmonary Tuberculosis (PTB) is a major public health issue globally, all age groups in community are at high risk to become get infected. Multidrug Resistant (MDR) and extensively drug resistant Mycobacterium tuberculosis is increasing day by day, which is an alarm for policy maker to improve the policy. Success of any Tuberculosis (TB) control programs depends on availability of accurate data regarding TB.
Aim: To know the resistant profile of Mycobacterium tuberculosis causing PTB by using Line Probe Assay (LPA).
Materials and Methods: It is a laboratory based observational study conducted in Department of Microbiology, IGIMS and TBDC, Bihar, India between January 2016 to December 2016. Sputum specimens were collected from all clinically suspected cases of TB. All specimens were subjected to smear microscopy, conventional culture and LPA. Standard operating protocols were followed for all the techniques.
Results: Totally 1772 sputum samples were collected during study period, positive for smear microscopy, conventional culture and LPA were 54%, 66% and 72% respectively. All smear positive isolates were positive by LPA, whereas only 840 by conventional culture. Among the smear negative isolates 309 were positive by LPA and 336 by conventional culture method. In LPA negative isolates 73 cases were positive by conventional culture methods. Out of 1272 LPA positive isolates 249 patient were diagnosed as PTB due to resistant strains. Among resistant isolates 60.64% were identified as both rifampicin and isoniazid resistant, whereas 21.68% and 17.67% was rifampicin and isoniazid mono resistant respectively.
Conclusion: We found that LPA can be a good diagnostic tool for early diagnosis of mono-resistant as well as MDR TB. To control the transmission of TB in community, control programs should be followed and early diagnosis of drug resistant TB must be ensured.
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