Original article / research
|
||||||
Prevalence and Antibiotic Resistance Pattern of Clinical Isolates of Enterococci from Blood Samples in A Tertiary Care Hospital, Jamnagar, Gujarat, India |
||||||
Correspondence
Address : Dr. Pooja A Kamaria, 160, Chitrakutdham Society, B/H Crystal Mall, Kalawad Road, Rajkot-360005, Gujrat, India. E-mail: Pooja.kamaria@gmail.com |
||||||
Introduction: In recent years, enterococci have become important nosocomial pathogens. Indiscriminate use of antibiotics, prolonged hospital stay, severity of illness and immunosuppression are responsible for nosocomial acquisition of drug resistant enterococci. Aim: To determine the prevalence and anti-microbial resistance pattern of enterococci in blood samples. Materials and Methods: This retrospective study was carried out in the Department of Microbiology, Shri M. P. Shah Government Medical College, Jamnagar, Gujarat from January 2014 to June 2014. A total of 540 blood samples were tested. The isolates were identified by grams staining, colony characteristics, catalase test, black color on bile esculin agar and tolerance to 6.5% NaCl. Antibiotic susceptibility test was done by Kirby-Bauer disc diffusion method for Penicillin (10 units), Azithromycin (30 mcg), Gentamicin (10 mcg), Ciprofloxacin (5 mcg), Tetracycline (30 mcg), Linezolid (30 mcg) and Vancomycin (30 mcg). Results: The prevalence rate of enterococci in blood samples was 6.48%. Antibiotic susceptibility tests showed high level resistance to commonly used antibiotics like Penicillin (100%), Azithromycin (85.71%), Gentamicin (100%), Ciprofloxacin (82.85%) & Tetracycline (77.14%) and spread to newer drugs like Linezolid (5.71%) and Vancomycin (8.57%). Conclusion: We conclude that enterococcal strains with high level of resistance to penicillin aminoglycosides and fluoroquinolones group of drugs. So there is urgent need for more rational and restricted use of antimicrobials and continuous monitoring, in order to minimize the selection and to prevent hospital spread of such strains. |
||||||
[ FULL TEXT ] | [ ] | ||||||