Original article / research
Oxidative Stress and Total Antioxidant Capacity in Renal Failure and Renal Calculi Patients-A Cross Sectional Study
Dr. Shivprasad S,
Assistant Professor, Department of Biochemistry,
Jawaharlal Nehru Medical College,
Belgaum, Karnataka, India.
Background: Oxidative stress has been the centre of focus in most of the research, and in the field of medicine it takes a special place especially in diseases associated with kidneys. Stimulation of renal tissue by factors like infection, proteinuria, food allergens, drugs, high glucose levels, and increased blood pressure reactive oxygen species are released resulting in tissue injury and progression of disease.
Aims: To determine the level of oxidative stress and the antioxidant defense in patients with CRF, ARF and renal calculi.
Methods & Materials: Patients between age group of 30 to 50 years were selected as participants of the study. 50 cases each of acute renal failure, chronic renal failure and renal calculi were taken along with 50 control samples. The blood was collected after obtaining written informed consent. 5 ml of blood was collected under aseptic precautions and all the parameters were evaluated by standard biochemical methods. Oxidative stress was measured in terms of MDA, a reliable indicator of lipid peroxidation product.
Statistical Analysis: Student “t” test (unpaired) – was used to calculate the statistical significance between cases and controls and also by Analysis of variance (ANOVA).
Results & Conclusion: Malondialdehyde representing the oxidative stress in patients of Chronic Renal Failure, Acute Renal Failure and renal calculi was high indicating the stress among those patients. Total antioxidant capacity was low among ARF and CRF in comparison with controls. Reactive oxygen species (ROS) formation is part of the nonspecific defense system of an organism. ROS may also affect almost all cells of the host organism, in particular at sites where there is active inflammation.
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