Original article / research
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Analytical Errors in End Point Biochemical Assays – Experimental Analysis and Importance |
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Correspondence
Address : Dr. P. K. Nigam, Biochemist, Department of Cardiology, King George’s Medical University, Lucknow-226003, India. Email: p_nigam1@yahoo.com Ph: 9235775577 |
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Introduction: Accurate and precise laboratory test results help immensely in the diagnosis and management of diseases. Though the analytical phase amounts to the least possible error, yet it is imperative to recognize and minimize the possible sources of errors to achieve good quality test results. In this article some points of precautions while running an end point assay have been highlighted. Material and Methods: The left over blood samples received in our laboratory for routine tests were used for this study. Two most simple and low cost tests i.e. glucose and urea were done. Result and Discussion: The importance of including a reagent blank and standard in every batch of assay has been demonstrated. In addition some other precautions such as using clear plasma/serum and mixing the thawed serum samples well, before analysis have also been discussed, ignoring which may give erroneous test results. Conclusion: Since the total analytical error is a cumulative effect of several factors it is better to be cautious at every step of the analysis to have least possible error. |
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